Acute Effect of Tobacco Snuff Consumption on Plasma Total Protein, Albumin, Globulin and Fasting Blood Sugar Level in Rats
Asian Journal of Medicine and Health,
This study was designed to investigate the effect of tobacco snuff consumption on total protein, albumin, globulin and fasting blood sugar level. Adult Wistar rats (12) weighing 150-300g were involved. They were divided into four groups; group A serving as control, while groups B, C and D served as the test groups. The rats were fed with varying doses of tobacco dust mixed with potash (tobacco snuff). At the end of the experiment, the animals were sacrificed and blood sample collected into lithium heparin container. Total protein (24.34 ± 4.10 g/dl), albumin (13.80 ± 4.66 g/dl), and fasting blood sugar were assayed and the results obtained showed statistically significant changes. However, fasting blood sugar (117.33 ± 38.50mg/dl) showed decrease that was not statistically significant in all the groups throughout the experiment. The results of this study suggest that tobacco snuff is toxic to the synthetic capacity of the liver and the observed changes were dose dependent.
- Tobacco snuff
- total protein
How to Cite
Omole OB, Ogunbanjo GA. Smokeless tobacco: Is it really safe? SA Fam Pract 2009; 51(4):292-295.
Okonkwo LO, Dada FL, Ugbor CI, Nwadike IG, Eze NO, Ozougwu CP. International Journal of Herbs and Pharmacological Research IJHPR. 2013a;2(3):29–35.
Bagchi M, Bagchi D, Adickes E, Stohs SJ. Chronic effects of smokeless tobacco extract on rat liver histopathology and production of HSP-90. J Environ Pathol Toxicol Oncol. 1995;14(2):61-8.
Bagchi M, Bagchi D, Hassoun EA, Stohs SJ. Subchronic effects of smokeless tobacco extract (STE) on hepatic lipid peroxidation, DNA damage and excretion of urinary metabolites in rats. Toxicology. 1998;127(1-3):29-38.
Critchley JA, Unal B. Health effects associated with smokeless tobacco: A systematic review. Thorax. 2003;58:435–443.
Lenz LG, Ramp WK, Galvin RJ, Pierce WM. Inhibition of cell metabolism by a smokeless tobacco extract: tissues and species specificity. Proc. Soc. Experimental Biol. Med. 1990;211-217.
Rajani S, Bhavna S, Ketan S, Partha R. Smokeless tobacco extract impairs iron homeostasis in rats and human hepatoma, HepG2 cells. Toxicological and Enviromental Chemistry. 2011; 93(5):1028-1044.
Sapundzhiev N, Werner JA. Nasal snuff: Historical review and health related aspects. J Laryngol Otol. 2003;117(9):686-91.
Wyckham RG. Smokeless tobacco in Canada: Deterring market development. Tob control. 1999;8:441-420.
Richard L, Crawford DVM. Animal welfare. In: Animal welfare act quick reference guides. animal welfare information centre with virginia-maryland regional college of veterinary medicine. Title 9 code of Federal Regulations (CFR), Chapter-1, subchapter A; 2012.
Nwaopara AO, Akpamu U, Izunya AM, Oaikhena GA, Okhiai O, Anyanwu LC, Idonije BO, Oyadonghon GP. The effect of Yaji-meat-sauce consumption on cerebellar neurons of white albino rats. Current Research Journal of Biological Sciences. 2011;3(4):308–312.
Bagchi M, Bagchi D, Hassoun EA, Stohs SJ. Smokeless tobacco induced increases in hepatic lipid peroxidation, DNA damage and excretion of urinary lipid metabolites. Internationl Journal of Experimental Pathology. 1994;75:197-202.
Ugbor CI, Eloka CCV, Okonkwo LO, Ugwu MC, Ogbodo LA. Tobacco induced priapism in wistar rat: A Case Report. International Journal of Herbs and Pharmacological Research. 2013a;2(1):1-5.
Faulkner WR, S Meites S. Selected methods for the small clinical chemistry laboratory. American Association for Clinical Chemistry Inc, USA. 1982;9.
Doumas BT, Watson WA, Briggs HG. Albumin standards and the measurement of serum albumin with bromocresol green. Clin. Chem. Acta. 1971;31:87-97.
Grant GH, et al. Amino acids and proteins. Fundamentals of clinical chemistry, Tieze NW. Editor, Third edition, WB Saunders Company Philadelphia USA. 1987;328-329.
Guyton A, Hall JE. Functional roles of the plasma proteins. Protein metabolism. Textbook of medical physiology, 11th edition. Saunders. Elsevier. 2006: Pp. 855.
Rothschild MA, Oratz M, Schreiber SS. New England J Med. 1972;286(15):816-821.
Raj Shrestha, Ashwini Kumar Nepal, Binod Kumar Lal Das, Basanta Gelal, Madhab Lamsal. Non-enzymatic Antioxidant Status and Biochemical Parameters in the Consumers of Pan Masala Containing Tobacco. Asian Pacific J Cancer Prev. 2012;13(9):4353-4356
Burtis CA, Ashwood ER, Bruns DE. Tietz textbook of clinical chemistry and molecular diagnostics, United States of America, Elsevier Saunders 4th Ed; 2006.
Ugbor CI, Okogun GRA, Okonkwo LO, Eze NC, Asogwa BE, Ebo JO, Maduagwuna GN, Ekoh SN. The effect of tobacco snuff consumption on liver enzymes. International Journal of Herbs and Pharmacological Research IJHPR. 2013b;2(2):20–27.
Pramod KA, Surender K, Chander MP, Kim V, Krishan LK. Smokeless tobacco impairs the antioxidant defense in liver, lung, and kidney of rats: Toxicol. Sci. 2006;89(2):547-553.
Gonzalez RA. Free radicals, oxidative stress and DNA metabolism in human cancer. Cancer Invest. 1999;17:376–377.
Merleb EM, Hoehn K. Human anatomy and Physiology Amazon.com books. (7Th edition); 2007.
Bagchi M, Bagchi D, Stohs SJ. In vitro effects of a smokeless tobacco extract on the production of reactive oxygen species by human oral epidermal cells and rat hepatic mitochondria and microsomes and peritoneal macrophages. Archives of Environmental Contamination and Toxicology. 1996;(3):418-422.
Oyeleke AO. Effect of consumption of “Kanwa” in food and water on certain physiological states in rats Nutrition. 1988;4:137-140.
Soladoye AO, Oyeleke DA. The effects of moderate intake of natron on growth rate and blood indices in rats. Proc. Int. Union Physiol Sci. 1989;5421.
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